Composite

Part:BBa_K759011:Design

Designed by: Ami Kawahata   Group: iGEM12_HokkaidoU_Japan   (2012-09-20)


P3HB producing construct


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 676
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 287
    Illegal NgoMIV site found at 402
    Illegal NgoMIV site found at 636
    Illegal NgoMIV site found at 948
    Illegal NgoMIV site found at 1227
    Illegal NgoMIV site found at 1640
    Illegal NgoMIV site found at 1707
    Illegal AgeI site found at 375
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3645


Design Notes

Nothing to remark.


Source

phaA and phaB was cloned from pGEM phbCAB which carries the gene of Ralstonia Eutropha.

References